Mapping ATP-dependent Activation at a σ54 Promoter
نویسندگان
چکیده
منابع مشابه
Mapping ATP-dependent activation at a sigma54 promoter.
The sigma(54) promoter specificity factor is distinct from other bacterial RNA polymerase (RNAP) sigma factors in that it forms a transcriptionally silent closed complex upon promoter binding. Transcriptional activation occurs through a nucleotide-dependent isomerization of sigma(54), mediated via its interactions with an enhancer-binding activator protein that utilizes the energy released in A...
متن کاملATP Binding to the σ54-Dependent Activator XylRTriggers a Protein Multimerization Cycle Catalyzed by UAS DNA
Finally, there are also cases Consejo Superior de Investigaciones Científicas in which the A domain plays only a small role in transcrip-28049 Madrid tional activity, either because its control depends on an Spain additional factor (such as the NifA protein, regulated by NifL) (Austin et al., 1990; Berger et al., 1994) or because the A domain does not exist as such (as is the case with Summary ...
متن کاملSURVEY AND SUMMARY Compilation and analysis of σ54-dependent promoter sequences
Promoters recognized by the RNA-polymerase with the alternative sigma factor σ54 (Eσ54) are unique in having conserved positions around –24 and –12 nucleotides upstream from the transcriptional start site, instead of the typical –35 and –10 boxes. Here we compile 186 –24/–12 promoter sequences reported in the literature and generate an updated and extended consensus sequence. The use of the ext...
متن کاملCRP-Cyclic AMP Dependent Inhibition of the Xylene-Responsive σ54-Promoter Pu in Escherichia coli
The expression of σ(54)-dependent Pseudomonas putida Pu promoter is activated by XylR activator when cells are exposed to a variety of aromatic inducers. In this study, the transcriptional activation of the P. putida Pu promoter was recreated in the heterologous host Escherichia coli. Here we show that the cAMP receptor protein (CRP), a well-known carbon utilization regulator, had an inhibitory...
متن کاملHigh-resolution nucleosome mapping reveals transcription-dependent promoter packaging.
Genome-wide mapping of nucleosomes has revealed a great deal about the relationships between chromatin structure and control of gene expression, and has led to mechanistic hypotheses regarding the rules by which chromatin structure is established. High-throughput sequencing has recently become the technology of choice for chromatin mapping studies, yet analysis of these experiments is still in ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 2006
ISSN: 0021-9258
DOI: 10.1074/jbc.m605731200